Novel strontium-doped bioactive glass nanoparticles enhance proliferation and osteogenic differentiation of human bone marrow stromal cells

The present study investigates a new family of bioactive glass nanoparticles with and without Sr-doping focusing on the influence of the nanoparticles on human bone marrow stromal cells (hBMSCs) in vitro. The bioactive glass nanoparticles were fabricated by flame spray synthesis and a particle diameter of 30–35 nm was achieved. Glass nanoparticles were undoped (BG 13-93-0Sr) or doped with 5 wt% strontium (Sr) (BG 13-93-5Sr) and used at concentrations of 10 and 100 �g/cm2 (particles per culture plate area), respectively. Cells were cultured for 14 days after which the samples were analysed regarding metabolic activity and expression of various bone-specific genes. Cell growth and morphology indicated the high cytocompatibility of the nanoparticulate bioactive glass. The presence of the nanoparticles enhanced cell growth compared to the plain polystyrene control group. At a concentration of 100 �g/cm2, Sr-doped particles led to significantly enhanced gene expression of osteocalcin, collagen type 1 and vascular endothelial growth factor. Thus, Sr-doped nanoparticles showing a dose-dependent increase of osteogenic differentiation in hBMSCs are a promising biomaterial for bone regeneration purposes. DOI: https://doi.org/10.1007/s11051-013-1780-5 Posted at the Zurich Open Repository and Archive, University of Zurich ZORA URL: https://doi.org/10.5167/uzh-86760 Accepted Version Originally published at: Strobel, L A; Hild, N; Mohn, D; Stark, W J; Hoppe, A; Gbureck, U; Horch, R E; Kneser, U; Boccaccini, A R (2013). Novel strontium-doped bioactive glass nanoparticles enhance proliferation and osteogenic differentiation of human bone marrow stromal cells. Journal of Nanoparticle Research, 15(7):1780. DOI: https://doi.org/10.1007/s11051-013-1780-5 Novel strontium-doped bioactive glass nanoparticles enhance proliferation and osteogenic differentiation of human bone marrow stromal cells L. A. Strobel; N. Hild, D. Mohn; W. J. Stark; A. Hoppe; U. Gbureck; R. E. Horch; U. Kneser ; A. R. Boccaccini* 1 Department of Plastic and Hand Surgery, University of Erlangen-Nuremberg Medical Center, Erlangen, Germany 2 Department of Hand, Plastic and Reconstructive Surgery, Burn Centre, BG Trauma Centre, Ludwigshafen/Rhine, Germany 3 Institute for Chemical and Bioengineering, Department of Chemistry and Applied Biosciences, ETH Zurich, Zurich, Switzerland 4 Department of Preventive Dentistry, Periodontology and Cariology, University of Zurich, Center of Dental Medicine, Zurich, Switzerland 5 Institute of Biomaterials, University of Erlangen-Nuremberg, Erlangen, Germany 6 Department for Functional Materials in Medicine and Dentistry, University of Würzburg, Würzburg, Germany Correspondence to: Prof. Dr.-Ing. habil. Aldo R. Boccaccini Institute of Biomaterials Department of Materials Science and Engineering University of Erlangen-Nuremberg Cauerstrasse 6 91058 Erlangen, Germany Tel.: +49 9131 85 28601 Fax: +49 9131 85 28602 Email: [email protected]